Journal: Journal for immunotherapy of cancer
Article Title: CHMP2A regulates broad immune cell-mediated antitumor activity in an immunocompetent in vivo head and neck squamous cell carcinoma model.
doi: 10.1136/jitc-2023-007187
Figure Lengend Snippet: Figure 7 EVs secreted by 4MOSC cell lines express mouse NKG2D ligands. (A) 4MOSC1 and 4MOSC2 WT and mCHMP2A KO derived EVs were collected and analyzed by flow cytometry for mouse NKG2D ligands mRae, mULBP, mH60 (blue histogram). Red histogram represents isotype. EVs, extracellular vehicles; KO, knock-out.
Article Snippet: Isolated EVs were incubated with primary staining with mRae (AF1136- SP, R&D Systems) mULBP (MAB2588- SP, R&D Systems), and mH60 (MAB1155- SP, R&D Systems) for 30 min on ice and washed with flow buffer.
Techniques: Derivative Assay, Flow Cytometry, Knock-Out